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Využití smart farming technologií při hospodaření na zemědělské půdě
Kovaříková, Jana
The bachelor thesis deals with the basic theoretical description of smart farming technologies or precision farming methods in agricultural land management. Precision farming is a trend that has been developing since the early 1990s. One of the elements of precision farming is locally targeted farming that works with soil heterogeneity and tillage using new technologies. Optimizing soil inputs (mineral fertilizers, pesticides, etc.) and applying them according to the current plant requirements is one of the goals of smart farming. Through precision farming agronomic practices, we can increase the efficiency of crop production, reduce the amount of mineral fertilizer inputs and improve both economic and environmental status. The aim of the presented work was to evaluate the variable application of essential nutrients (P and K) on selected plots managed in a conventional way using PREFARM. The experiment itself was carried out in the agricultural company AGROSUMAK a.s., The practical part includes a comparison of lump and variable application of potassium salt (K2O source) and triple superphosphate (P2O5 source) on three experimental plots. Data on plot heterogeneity were provided by AGROSUMAK a.s., which uses the PREFARM system. In the conclusion of the present work, the results are discussed and compared from economic, environmental and agronomic points of view.
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Mapování heterogenity stanoviště pomocí metod a technologií používaných v systému precizního zemědělství
Šmoldas, Radomír
The master thesis "Mapping habitat heterogeneity using methods and technologies used in the system of precision agriculture" deals with the heterogeneity of fields in the selected farm Tršická zeměděská a.s. The thesis begins with literature review which has knowledge critical for practical part of the thesis. At the beginning precision farming and its goals are briefly described. Furthermore, the literature review deals with the issue of mapping the heterogeneity of fields and how to monitor it. The following section discusses global navigation systems. Specifically, what versions do we have and their contribution to precision agriculture. Literature reviews ends on the chapters of remote sensing and geographic information systems. The thesis continues with the practical part, which deals with the heterogeneity of selected fields in the farm Tršická zemědělská a. s. First, however, the natural and production conditions and the equipment of the farm are described. After that the supply of macroelements and pH was monitored on the selected fields, and maps of the supply were created in the ArcGIS program. Subsequently, these maps were compared with the results from 2021. Finally, maps of the recommended rate of variable fertilization of the fields were created for the model crop and desired yield.
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Influence of environment in host cells on heterogeneity of virulence factors expression in Salmonella
Mathéová, Paulína ; Černý, Ondřej (advisor) ; Konopásek, Ivo (referee)
Bacteria of the genus Salmonella are one of the most important human pathogens. Salmonella expresses several virulence factors, including the type three secretion system encoded on the Salmonella pathogenicity island 2 (SPI-2 T3SS) and its effector proteins. SPI- 2 T3SS effector proteins allow the bacteria to colonize host cells, persist there and use them as a Trojan horse for migration throughout the host organism. Although the function of the SPI- 2 T3SS is essential for the bacterial population during systemic infection, some bacteria do not express this virulence factor and benefit from its expression by other members of the population. Similar heterogeneity in expression of other virulence factors has been shown to be crucial for their function. This work focuses on the heterogeneity of expression of selected SPI-2 T3SS effector proteins under the influence of host factors (pH, ROS, RNS) affecting bacteria during intracellular infection of macrophages. Using a fluorescent reporter system suitable for flow cytometry the expression of selected effector proteins was detected at the level of individual bacteria. The obtained data show, that individual bacteria respond differently to the decrease in pH in their vicinity. Part of the bacterial population starts to express the studied effector...
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Heterogeneity of antigen-presenting cells in the thymus and its relevance for the establishment of central tolerance
Sýkora, Vojtěch ; Filipp, Dominik (advisor) ; Černý, Jan (referee)
The crucial function of the thymus is the establishment of central tolerance. In this process, developing T-cells are tested for their self-reactivity, since self-reactive T-cells might cause the autoimmunity if they would escape from the thymus to the periphery. Many thymic antigen-presenting cells are essential for establishment of central tolerance. Their role is to present self-antigens to the developing T-cells. Such presentation is capable to reveal the self-reactive potential of T-cells which can be then directly removed or deviated into suppressive T-regulatory cells. In the last several years, a high level of heterogeneity has been described among the thymic antigen-presenting cells and the molecular mechanisms that govern their functions towards enforcement of tolerance began to be uncovered. This thesis summarises recent knowledge in the field of heterogeneity of the thymic antigen-presenting cells and its relevance for establishment of the central tolerance, with the major focus on conventional dendritic cells and post-AIRE medullary thymic epithelial cells. This thesis also outlines recent advances in understanding of functional mechanisms and regulations of maturation of the antigen-presenting cells.
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Social isolation during Covid-19 pandemic: the perspective of the elderly living alone
Picková, Šárka ; Povolná, Pavla (advisor) ; Lejsal, Matěj (referee)
This thesis concerns the problem of social isolation in older adults living alone. It authentically captures the unique experience of older people during the Covid-19 pandemic. The aim of this work is to understand whether older people think their needs changed during the period of Covid-19 pandemic in comparison with the time before it, and moreover to formulate recommendations for field workers providing health and social services, general practitioners and municipalities with extended powers. This thesis is processed by the method of interpretive phenomenological analysis. The theoretical part presents a view of social isolation in older adults, risk factors leading to it and possibilities of prevention through various social institutions. It focuses not only on the period of the Covid-19 pandemic when the risk of social isolation was significantly influenced by the emergency measures against the spread of the diseases but also on the period before its onset. The empirical part presents the issue of social isolation of older adults during the Covid-19 pandemic from the perspective of the adults themselves. The research survey is supplemented with the experience of experts working with the target group of older adults. The real experience of participants in the research survey shows that the...
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Habitat dependence of persistence of clonal connection in plants
Martincová, Nina ; Weiser, Martin (advisor) ; Koubek, Tomáš (referee)
The review is about the role of environment to the longevity of connection in clonal plants. The first part is dealing with theoretical models, which describe the interaction of plants with different strategies in longevity of clonal connection in a model habitat. Subsequently, it analyses concrete attributes of the environment - heterogeneity, carrying capacity, amount of disturbances and competition. This part focuses more thoroughly on their impact on persistence of clonal connection and strategy of clonal reproduction in general. The last part refers to some other phenomena which could influence the length of persistence of a clonal connection.
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Influence of understory light on diversity and composition of forest vegetation in the České Středohoří Mts.
Macek, Martin ; Kopecký, Martin (advisor) ; Černý, Tomáš (referee)
Light is essential component of forest ecosystem, as limited light availability in forest-floor restricts herb and tree seedlings growth. Importance of light availability for maintaining plant species diversity and community structure wasn't sufficiently evaluated in the Czech Republic. This study focus on the ecological relevance of light availability on both landscape and local scale. Relationship of light to cover, diversity and species composition was quantified in the thesis. Dominant tree species affected light availability and its spatial variability. Light availability was along the soil reaction and slope most influential factor for large-scale species diversity. Within-site light variability affected plant diversity more than soil reaction variability. Measuring light by means of hemispherical photography seems to be an appropriate for studies like this. Light availability influnence on community compostition is weak in comparison with other environmental gradients.
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Heterogeneity of expression of virulence factors of Salmonella.
Mathéová, Paulína ; Černý, Ondřej (advisor) ; Lichá, Irena (referee)
The emergence of phenotypically heterogeneous individuals within an isogenic bacterial population is considered to be an important adaptation to the host environment. It allows survival of some bacterial subpopulations under diverse stress conditions caused by the host immune system, the emergence of a "division of labor" and cooperation between individual bacteria. Bacteria of the genus Salmonella are important pathogens in humans and livestock. Many of the key virulence factors of Salmonella are heterogeneously expressed. The phenotypic diversity of individual bacteria allows certain individuals to escape the host's immune system and ensure that the gene pool is preserved to future generations. In case when change in conditions causes complete extinction of part of the Salmonella population from the environment, the remaining individuals are able to restore the size of the population and phenotypic diversity, after overcoming unfavourable conditions. This work summarises the knowledge about heterogeneity of expression of virulence factors of Salmonella and the characteristics of individual subpopulations in different environmental conditions. Keywords: Salmonella, heterogeneity, virulence factors, bet-hedging, division-of-labor, bacterial subpopulations.
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The CSSD crisis in 2013-2018
Kusbach, Ondřej ; Perottino, Michel (advisor) ; Stauber, Jakub (referee)
In the past, the Czech social democratic party was one of the most successful political formations in the Czech Republic, however, since 2013 the party is dealing with a loss of electoral support and will face the challenge of staying in the Chamber of Deputies in the upcoming years. This diploma thesis is focused on the course of events in the party during the years 2013-2018 and examines the causes of why did a party, which used to get 30% of votes and used to dominate the regional political level, got into both electoral and intra-party crisis. The first chapter of the thesis describes four main theoretical concepts, which are used for analysis of the party processes. Subsequently, the thesis is divided into two analytical chapters. The first one concerns the events, which the party went through during the selected five year period. Its content is a factual description, which is accompanied by a closer look on intra-party disputes and on the development of party electoral support. Intra-party disputes are analyzed from a media point of view with an emphasis on how the media reflected them, and the development of party electoral support is analyzed within the framework of selected time periods with regard to the impact of intra-party disputes. The second analytical chapter addresses selected...
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